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paper n a a375 melanoma cells atcc crl 1619 a375  (ATCC)


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    ATCC paper n a a375 melanoma cells atcc crl 1619 a375
    Paper N A A375 Melanoma Cells Atcc Crl 1619 A375, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a375+atcc/A-375/pm42143019-247-148-153
    Average 99 stars, based on 5224 article reviews
    paper n a a375 melanoma cells atcc crl 1619 a375 - by Bioz Stars, 2026-09
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    Article Title: Functional nutrient-genetic profiling reveals biotin and FBXW7 are essential to bypass glutamine addiction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data 13 C- and 15 N-glutamine isotopic profiling in K562; 13 C-pyruvate isotopic profiling in primary CD8 + T cells This study MTBLS13799 13 C-pyruvate isotopic profiling in K562 This study 14002048 Multiple pathway targeted analysis of polar metabolites This study 14002048 Mass spectrometry data This study PXD057389 Spheroid imaging data This study https://www.doi.org/10.17632/328g7zyt9d.1 K562 RNAseq Slavoff et al. 81 GSE34740 Transcription factor ChIP-seq in K562 ENCODE 68 MYC(ENCFF801QJW), MNT(ENCFF611RGV), SIN3A(ENCFF085NLG) H3K27ac ChIP-seq in K562 ENCODE 68 ENCFF381NDD Experimental models: Cell lines Human: K562 American Type Culture Collection (ATCC) Cat# CCL-243; RRID: CVCL_0004 Human: HEK293T ATCC Cat# CRL-3216; RRID: CVCL_0063 Human: HeLa ATCC Cat# CCL-2; RRID: CVCL_0030 Human: MCF-7 ATCC Cat# HTB-22; RRID: CVCL_0031 Human: SW480 ATCC Cat# CCL-228; RRID: CVCL_0546 Human: UACC-257 Frederick Cancer Division of Cancer Treatment and Diagnostic (DCTD) RRID: CVCL_1779 Human: U-2 OS ATCC Cat# HTB-96; RRID: CVCL_0042 Human: A375 ATCC Cat# CRL-1619; RRID: CVCL_0132 Mouse: MC38 Sigma-Aldrich Cat# SCC172; RRID: CVCL_B288 Mouse: B16-F10 ATCC Cat# CRL-6475; RRID: CVCL_0159 Mouse: MycER mouse embryonic fibroblast Wise et al. 9 ; Linder et al. 19 N/A Oligonucleotides sgRNAs, see Table S9 Integrated DNA Technologies N/A ChIP primers, see Table S9 Integrated DNA Technologies N/A Random hexamers ThermoFisher Cat# N8080127 TaqMan Gene Expression Assay, human PC, FAM-MGB Life Technologies Cat# Hs01085875_g1 Taqman Gene Expression Assay, human TBP, VIC-MGB Life Technologies Cat# Hs00427620_m1 Recombinant DNA pLX304-GFP-V5 Kim et al. 82 Addgene, Cat# 193687; RRID: Addgene_193687 pLX304-PC Wiemann et al. 83 DNAsu, Cat# HsCD00436386 pWPI-Neo-GFP-3xFLAG Jourdain et al. 84 Addgene, Cat# 201639; RRID: Addgene_201639 pWPI-SLC7A11-HA Jourdain et al. 84 Addgene, Cat# 201643; RRID: Addgene_201643 pMXS-SLC1A3 Birsoy et al. 30 Addgene, Cat# 72873; RRID: Addgene_72873 pLVX-TetOne-Puro-GFP-3xFLAG This study N/A pLVX-TetOne-Puro-LbNOX Titov et al. 31 and Choe et al. 85 Addgene, Cat# 234984; RRID: Addgene_234984 pTwist-Lenti-CMV-BSD-GFP-3xFLAG This study Twist Bioscience pTwist-Lenti-CMV-BSD-PC-WT This study Twist Bioscience pTwist-Lenti-CMV-BSD-PC-K1144R This study Twist Bioscience pTwist-Lenti-CMV-Puro-FBXW7-WT This study Twist Bioscience pTwist-Lenti-CMV-Puro-FBXW7-R465C This study Twist Bioscience (Continued on next page) Molecular Cell 86, 901–916.e1–e10, March 5, 2026 e3

    Derivative Assay:

    Article Title: Coexpression of MTH1 and PMS2 Is Associated with Advanced Disease and Disease Progression after Therapy in Melanoma.
    Article Snippet: .. A375VR4, a BRAFV600E mutated BRAF inhibitor (vemurafenib)eresistant cell line was derived in-house from A375 ATCC (Manassas, VA) (Azimi et al., 2017). .. NRAS mutant SkMel2 (Q61R) was purchased from ATCC, and the BRAF/NRAS wild-type cell line ESTDAB105 was purchased from European Searchable Tumor Line Database and Cell Bank.

    Recombinant:

    Article Title: Cortical organoid-derived models of the melanoma brain metastatic niche enable prioritization of cancer-targeting drugs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ethanol VWR International Cat# 85651 BD Cytofix/Cytoperm Fixation and Permeabilization Solution BD Biosciences Cat# 554722 FluoroFix Buffer BioLegend Cat# 422101 Intracellular Staining Permeabilization Wash Buffer BioLegend Cat# 421002 Anti-cancer approved drug library Hö lzel Diagnostika Handels GmbH Cat# L2110-1-TM Temsirolimus Biomol Cat# TGM-T2145 GSK343 Biomol Cat# TGM-T6059 Bortezomib Hö lzel Diagnostika Handels GmbH Cat# TMO-T2399 GrowDex-T Revvity Cat# 200103002 Selinexor Selleckchem Cat# S7252 DEBIO-0932 MedChemExpress Cat# HY-13469 Carbamazepine Sigma-Aldrich Cat# C4024 Atenolol Sigma-Aldrich Cat# A7655 Verapamil hydrochloride Sigma-Aldrich Cat# V4629 Fluorescein sodium salt Sigma-Aldrich Cat# F6377 Carbutamid AbbVie In-house synthesis Water with 0.1% (v/v) formic acid VWR International Cat# 84867.290 Acetonitrile VWR International Cat# 83640.290 Ammonium bicarbonate Sigma-Aldrich Cat# 5.33005 Ammonium hydroxide Honeywell International Cat# 30501-1L Critical commercial kits mTeSR Plus STEMCELL Technologies Cat# 100-0276 Venor GeM Sample Preparation Kit Minerva Biolabs Cat# 56-1050 Venor GeM OneStep Kit Minerva Biolabs Cat# 11-8100 STEMdiff Dorsal Forebrain Organoid Differentiation Kit STEMCELL Technologies Cat# 08620 STEMdiff Neural Organoid Maintenance Kit STEMCELL Technologies Cat# 100-0120 UltraView Universal DAB Detection Kit Roche Cat# 760-500 QIAshredder QIAGEN Cat# 79656 RNeasy Micro Kit QIAGEN Cat# 74004 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1632 TaqMan Primer Assay TBP Thermo Fisher Scientific TaqMan Assay ID: Hs00427620_m1 TaqMan Primer Assay MAP2 Thermo Fisher Scientific TaqMan Assay ID: Hs00258900_m1 TaqMan Primer Assay PAX6 Thermo Fisher Scientific TaqMan Assay ID: Hs01088114_m1 TaqMan Primer Assay VGLUT1 Thermo Fisher Scientific TaqMan Assay ID: Hs00220404_m1 TaqMan Primer Assay BSN Thermo Fisher Scientific TaqMan Assay ID: Hs01109152_m1 SYLGARD 184 Silicone Elastomer Kit Dow N/A ATPlite 1step Luminescence Assay System Revvity Cat# 6016736 Caspase-Glo® 3/7 3D Assay Promega Cat# G8981 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9682 Human CXCL10/IP-10 ELISA Kit - Quantikine Bio-Techne Cat# DIP100 Deposited data Bulk RNA seq dataset This paper GEO: GSE293348 Experimental models: Cell lines Human iPSC line: WISCi004-B WiCell Research Institute RRID: CVCL_C437 (Continued on next page) Cell Reports Methods 5, 101236, December 15, 2025 e3 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Human iPSC line: ZIPi013-B ZIP gGmbH; Tandon et al. 62 RRID: CVCL_UF44 Human iPSC line: HHUUKDi009-A HHU Dü sseldorf; Lorenz et al. 63 RRID: CVCL_B3T9 Human melanoma cell line: A375 ATCC Cat# CRL-1619; RRID: CVCL_0132 Human melanoma cell line: MelDCC3 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC8 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC11 University of Regensburg/ Fraunhofer ITEM-R N/A Recombinant DNA pRRL-CMV-GFP-puro Swiss Institute of Cancer Research N/A LeGO-T2 University Medical Center Hamburg- Eppendorf Addgene, Cat#27342; RRID: Addgene_27342 Software and algorithm Kaleido software Revvity N/A VisiSens scientifical software PreSens https://www.presens.de/de/produkte/ detail/visisens-scientifical-software ImageJ 1.54 NIH https://imagej.net; RRID: SCR_003070 Imaris 10.0.0 Oxford Instruments http://www.bitplane.com/imaris/imaris; RRID: SCR_007370 Harmony Revvity https://www.perkinelmer.com/product/ harmony-5-1-office-hh17000012; RRID: SCR_023543 Patch Master V2x80 HEKA Elektronik https://www.heka.com/downloads/ downloads_main.html#down_patchmaster Origin Pro OriginLab http://www.originlab.com/index. aspx?go=PRODUCTS/Origin; RRID: SCR_014212 STAR v.2.4 Dobin et al. 64 http://code.google.com/p/rna-star/; RRID: SCR_004463 featureCounts v.1.5.1 Liao et al. 65 http://bioinf.wehi.edu.au/featureCounts/; RRID: SCR_012919 DESeq2 v.3.12 Love et al. 66 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID: SCR_015687 biomaRt v.4.0 Durinck et al. 67 https://bioconductor.org/packages/ biomaRt/; RRID: SCR_019214 clusterProfiler Yu et al. 68 http://bioconductor.org/packages/release/ bioc/html/clusterProfiler.html; RRID: SCR_016884 STRING Global Biodata Coalition and ELIXIR http://string.embl.de/; RRID: SCR_005223 FlowJo BD Biosciences https://www.flowjo.com/solutions/flowjo; RRID: SCR_008520 Analyst 1.7.2 Sciex https://sciex.com/products/software/ analyst-software GraphPad Prism 10 GraphPad http://www.graphpad.com/; RRID: SCR_002798 Other 6-well plates Greiner Bio-One Cat# 657-160 AggreWell 800 STEMCELL Technologies Cat# 34815 96-well ultra-low attachment plates Corning Cat# 7007 (Continued on next page) e4 Cell Reports Methods 5, 101236, December 15, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Nunc 6-well plates Thermo Scientific Cat# 140675 ThinCert cell culture inserts Greiner Bio-One Cat# 662-641 Corning mini bioreactor Sigma-Aldrich Cat# CLS431720 24-well plates Greiner Bio-One Cat# 662160 35 mm Petri dish Thermo Scientific Cat# 130180 Echo Qualified 384-well plates Labcyte Cat# 001-16128 384-well plates Greiner Bio-One Cat# 781091 96-well cell culture microplate, CELLSTAR, black Greiner Bio-One Cat# 655090 96-well cell culture microplate, CELLSTAR, white Greiner Bio-One Cat# 655083 Breathseal sealer Greiner Bio-One Cat# 676050 Multi-channel micropipettes Brand N/A Cell culture warming plate Labotect N/A EnSight multimode plate reader Revvity N/A Oxygen-sensor foils PreSens Cat# SF-RPSu4 VisiSens TD PreSens N/A CS-Mount Lens ArduCam Cat# CS2325ZM01 Silicon glue RS Components N/A Muvi SPIM Bruker N/A ASP300S tissue processor Leica biosystems N/A Antigen Retriever 2100 Aptum N/A Operetta CLS High Content Imaging System Revvity N/A Ventana BenchMark XT autostainer Ventana N/A Zeiss Axioscope 5 microscope Zeiss N/A Axiocam 208 color camera Zeiss N/A NanoDrop 1000 spectrophotometer Thermo Fisher Scientific N/A QuantStudio 6 Flex Real-Time-PCRSystem Applied Biosystems N/A EPC 10 USB System with Red Star Headstage Warner Instruments Cat# 89-5273 Eclipse FN1 microscope Nikon N/A LYNX microscopy tissue processor Reichert-Jung N/A Side-mounted 2kx2k camera TRS TRO ̈ NDLE Restlichtverstä rkersysteme N/A LEO 912AB electron-microscope ZEISS N/A Ultracut S Microtome Reichert-Jung N/A Diamond knives DiATOME N/A Autosamdri-815 Tousimi N/A Goldpalladium Leica Microsystems Cat# EM ACE 200 Crossbeam 550 Zeiss N/A FACSymphony A3 BD Biosciences N/A Gallios 10C 3L Flow Cytometer Beckmann Coulter N/A Echo 550 liquid handler Labcyte N/A MultiDrop Thermo Fisher Scientific N/A Standard Tube Cassettes Steinle Labtechnology N/A EnVision Multimode Plate Reader Revvity N/A cellZscope nanoAnalytics N/A Infinite M1000 Pro TECAN N/A MTS4 microtiter shaker IKA N/A (Continued on next page) Cell Reports Methods 5, 101236, December 15, 2025 e5 OPEN ACCESS

    Article Title: CDC42-effector interaction inhibitors alter patterns of vessel arborization in skin and tumors in vivo
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal CD31 (PECAM-1) Brillian Violet 421 eBioscience Cat# 404-0311-82; RRID:AB_2929070 Monoclonal CD140a (PDGFRA) PE eBioscience Cat# 12-1401-81; RRID:AB_657615 Fixable Viability Dye eFlour 780 eBioscience Cat# 65-0865-14 Mouse CD45 R&D Cat# MAB114; RRID: AB_357485 Monoclonal Lyve1 (ALY7) eBioscience Cat# 14-0443-82; RRID:AB_1633414 Phospho-S6 Ribosomal Protein (Ser235/236) − 488 Cell Signaling Cat# 4803; RRID: AB_916158 S6 Ribosomal Protein − 488 Cell Signaling Cat# 5317; RRID:AB_10694920 Anti-mouse CD31 eBiosciences Cat# 553370; RRID:AB_394816 Monoclonal CD34 (RAM34) eBioscience Cat# 14034182; RRID:AB_467210 Lectin (LEL, TL) DyLight 649 Vector Lab Cat# DL-1178-1 Biological samples BRAFV600K,PTPN11N58S National Cancer Institute 563396-261-R Chemicals, peptides, and recombinant proteins Linifanib (ABT869) MedChemExpress Cat# HY-50751 Vemurafenib (PLX4032) SellekChem Cat# S1267 CASIN MedChemExpress Cat# HY-12874 Deposited data RNA sequence data This paper GEO: GSE298488 Experimental models: Cell lines Human: WM3248 Coriell Medical Institute Cat# WC00081; RRID:CVCL_6798 Human: A375 ATCC Cat# CRL-1619; RRID:CVCL_0132 Human: cell-derived endothelial cells Lab of Hughes Hachey et al.56 Human: Lung Fibroblasts Lonza Cat# CC-2512 Experimental models: Organisms/strains NOD.Cg-PrkdcScid Jackson RRID:IMSR_JAX:005557 C57BL/6J Jackson RRID:IMSR_JAX:000664 Software and algorithms ImageJ National Institute of Health Bethesda USA https://imagej.nih.gov/ij/ AngioTool National Cancer Institute Zudire et al.51 neuTube Howard Hughes Medical Institute Feng L et al.52 REAVER BSD 3.0 open source Corliss et al.58 PANTHER https://pantherdb.org/ Mi H et al.75 STRINGv12 https://string-db.org/ Szklarczyk D et al.76 RUVSeq www.bioconductor.org/ Risso D et al.77 Samtools/1.15.1 Lab of Trapnell Li H et al.78 Prism 10.4.1 GraphPad www.graphpad.com/ HISAT2 v2.2.1 Lab of Trapnell Kim et al.79 FlowJo BD Bioscience https://www.flowjo.com/ MATLAB MathWorks www.mathworks.com/ products/matlab.html iScience 28, 112971, July 18, 2025 e1 ..

    Software:

    Article Title: Cortical organoid-derived models of the melanoma brain metastatic niche enable prioritization of cancer-targeting drugs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ethanol VWR International Cat# 85651 BD Cytofix/Cytoperm Fixation and Permeabilization Solution BD Biosciences Cat# 554722 FluoroFix Buffer BioLegend Cat# 422101 Intracellular Staining Permeabilization Wash Buffer BioLegend Cat# 421002 Anti-cancer approved drug library Hö lzel Diagnostika Handels GmbH Cat# L2110-1-TM Temsirolimus Biomol Cat# TGM-T2145 GSK343 Biomol Cat# TGM-T6059 Bortezomib Hö lzel Diagnostika Handels GmbH Cat# TMO-T2399 GrowDex-T Revvity Cat# 200103002 Selinexor Selleckchem Cat# S7252 DEBIO-0932 MedChemExpress Cat# HY-13469 Carbamazepine Sigma-Aldrich Cat# C4024 Atenolol Sigma-Aldrich Cat# A7655 Verapamil hydrochloride Sigma-Aldrich Cat# V4629 Fluorescein sodium salt Sigma-Aldrich Cat# F6377 Carbutamid AbbVie In-house synthesis Water with 0.1% (v/v) formic acid VWR International Cat# 84867.290 Acetonitrile VWR International Cat# 83640.290 Ammonium bicarbonate Sigma-Aldrich Cat# 5.33005 Ammonium hydroxide Honeywell International Cat# 30501-1L Critical commercial kits mTeSR Plus STEMCELL Technologies Cat# 100-0276 Venor GeM Sample Preparation Kit Minerva Biolabs Cat# 56-1050 Venor GeM OneStep Kit Minerva Biolabs Cat# 11-8100 STEMdiff Dorsal Forebrain Organoid Differentiation Kit STEMCELL Technologies Cat# 08620 STEMdiff Neural Organoid Maintenance Kit STEMCELL Technologies Cat# 100-0120 UltraView Universal DAB Detection Kit Roche Cat# 760-500 QIAshredder QIAGEN Cat# 79656 RNeasy Micro Kit QIAGEN Cat# 74004 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1632 TaqMan Primer Assay TBP Thermo Fisher Scientific TaqMan Assay ID: Hs00427620_m1 TaqMan Primer Assay MAP2 Thermo Fisher Scientific TaqMan Assay ID: Hs00258900_m1 TaqMan Primer Assay PAX6 Thermo Fisher Scientific TaqMan Assay ID: Hs01088114_m1 TaqMan Primer Assay VGLUT1 Thermo Fisher Scientific TaqMan Assay ID: Hs00220404_m1 TaqMan Primer Assay BSN Thermo Fisher Scientific TaqMan Assay ID: Hs01109152_m1 SYLGARD 184 Silicone Elastomer Kit Dow N/A ATPlite 1step Luminescence Assay System Revvity Cat# 6016736 Caspase-Glo® 3/7 3D Assay Promega Cat# G8981 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9682 Human CXCL10/IP-10 ELISA Kit - Quantikine Bio-Techne Cat# DIP100 Deposited data Bulk RNA seq dataset This paper GEO: GSE293348 Experimental models: Cell lines Human iPSC line: WISCi004-B WiCell Research Institute RRID: CVCL_C437 (Continued on next page) Cell Reports Methods 5, 101236, December 15, 2025 e3 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Human iPSC line: ZIPi013-B ZIP gGmbH; Tandon et al. 62 RRID: CVCL_UF44 Human iPSC line: HHUUKDi009-A HHU Dü sseldorf; Lorenz et al. 63 RRID: CVCL_B3T9 Human melanoma cell line: A375 ATCC Cat# CRL-1619; RRID: CVCL_0132 Human melanoma cell line: MelDCC3 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC8 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC11 University of Regensburg/ Fraunhofer ITEM-R N/A Recombinant DNA pRRL-CMV-GFP-puro Swiss Institute of Cancer Research N/A LeGO-T2 University Medical Center Hamburg- Eppendorf Addgene, Cat#27342; RRID: Addgene_27342 Software and algorithm Kaleido software Revvity N/A VisiSens scientifical software PreSens https://www.presens.de/de/produkte/ detail/visisens-scientifical-software ImageJ 1.54 NIH https://imagej.net; RRID: SCR_003070 Imaris 10.0.0 Oxford Instruments http://www.bitplane.com/imaris/imaris; RRID: SCR_007370 Harmony Revvity https://www.perkinelmer.com/product/ harmony-5-1-office-hh17000012; RRID: SCR_023543 Patch Master V2x80 HEKA Elektronik https://www.heka.com/downloads/ downloads_main.html#down_patchmaster Origin Pro OriginLab http://www.originlab.com/index. aspx?go=PRODUCTS/Origin; RRID: SCR_014212 STAR v.2.4 Dobin et al. 64 http://code.google.com/p/rna-star/; RRID: SCR_004463 featureCounts v.1.5.1 Liao et al. 65 http://bioinf.wehi.edu.au/featureCounts/; RRID: SCR_012919 DESeq2 v.3.12 Love et al. 66 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID: SCR_015687 biomaRt v.4.0 Durinck et al. 67 https://bioconductor.org/packages/ biomaRt/; RRID: SCR_019214 clusterProfiler Yu et al. 68 http://bioconductor.org/packages/release/ bioc/html/clusterProfiler.html; RRID: SCR_016884 STRING Global Biodata Coalition and ELIXIR http://string.embl.de/; RRID: SCR_005223 FlowJo BD Biosciences https://www.flowjo.com/solutions/flowjo; RRID: SCR_008520 Analyst 1.7.2 Sciex https://sciex.com/products/software/ analyst-software GraphPad Prism 10 GraphPad http://www.graphpad.com/; RRID: SCR_002798 Other 6-well plates Greiner Bio-One Cat# 657-160 AggreWell 800 STEMCELL Technologies Cat# 34815 96-well ultra-low attachment plates Corning Cat# 7007 (Continued on next page) e4 Cell Reports Methods 5, 101236, December 15, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Nunc 6-well plates Thermo Scientific Cat# 140675 ThinCert cell culture inserts Greiner Bio-One Cat# 662-641 Corning mini bioreactor Sigma-Aldrich Cat# CLS431720 24-well plates Greiner Bio-One Cat# 662160 35 mm Petri dish Thermo Scientific Cat# 130180 Echo Qualified 384-well plates Labcyte Cat# 001-16128 384-well plates Greiner Bio-One Cat# 781091 96-well cell culture microplate, CELLSTAR, black Greiner Bio-One Cat# 655090 96-well cell culture microplate, CELLSTAR, white Greiner Bio-One Cat# 655083 Breathseal sealer Greiner Bio-One Cat# 676050 Multi-channel micropipettes Brand N/A Cell culture warming plate Labotect N/A EnSight multimode plate reader Revvity N/A Oxygen-sensor foils PreSens Cat# SF-RPSu4 VisiSens TD PreSens N/A CS-Mount Lens ArduCam Cat# CS2325ZM01 Silicon glue RS Components N/A Muvi SPIM Bruker N/A ASP300S tissue processor Leica biosystems N/A Antigen Retriever 2100 Aptum N/A Operetta CLS High Content Imaging System Revvity N/A Ventana BenchMark XT autostainer Ventana N/A Zeiss Axioscope 5 microscope Zeiss N/A Axiocam 208 color camera Zeiss N/A NanoDrop 1000 spectrophotometer Thermo Fisher Scientific N/A QuantStudio 6 Flex Real-Time-PCRSystem Applied Biosystems N/A EPC 10 USB System with Red Star Headstage Warner Instruments Cat# 89-5273 Eclipse FN1 microscope Nikon N/A LYNX microscopy tissue processor Reichert-Jung N/A Side-mounted 2kx2k camera TRS TRO ̈ NDLE Restlichtverstä rkersysteme N/A LEO 912AB electron-microscope ZEISS N/A Ultracut S Microtome Reichert-Jung N/A Diamond knives DiATOME N/A Autosamdri-815 Tousimi N/A Goldpalladium Leica Microsystems Cat# EM ACE 200 Crossbeam 550 Zeiss N/A FACSymphony A3 BD Biosciences N/A Gallios 10C 3L Flow Cytometer Beckmann Coulter N/A Echo 550 liquid handler Labcyte N/A MultiDrop Thermo Fisher Scientific N/A Standard Tube Cassettes Steinle Labtechnology N/A EnVision Multimode Plate Reader Revvity N/A cellZscope nanoAnalytics N/A Infinite M1000 Pro TECAN N/A MTS4 microtiter shaker IKA N/A (Continued on next page) Cell Reports Methods 5, 101236, December 15, 2025 e5 OPEN ACCESS

    Article Title: Targeting USP2 regulation of VPRBP-mediated degradation of p53 and PD-L1 for cancer therapy
    Article Snippet: .. Methodology Sample preparation Instrument Software Cell population abundance EMT6 ATCC Cat# CRL-2755 293T ATCC Cat# CRL-3216; H1299 ATCC Cat# CRL-5803; U2OS ATCC Cat# HTB-96; CAL33 Creative Bioarray Cat# CSC-C0479; HUCCT1 Creative Bioarray Cat# CSC-C9200W; SKBR3 ATCC HTB-30; A549 ATCC Cat# CCL-185; A375 ATCC Cat# CRL-1619; H460 ATCC Cat# HTB-177; SKBR3 ATCC Cat# HTB-30; MDA-MB-435 ATCC Cat# HTB-129; MDA-MB-231 ATCC Cat# HTB-26; RM-1 ATCC Cat# CRL-3310; All cell lines were not authenticated. ..

    Sequencing:

    Article Title: CDC42-effector interaction inhibitors alter patterns of vessel arborization in skin and tumors in vivo
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal CD31 (PECAM-1) Brillian Violet 421 eBioscience Cat# 404-0311-82; RRID:AB_2929070 Monoclonal CD140a (PDGFRA) PE eBioscience Cat# 12-1401-81; RRID:AB_657615 Fixable Viability Dye eFlour 780 eBioscience Cat# 65-0865-14 Mouse CD45 R&D Cat# MAB114; RRID: AB_357485 Monoclonal Lyve1 (ALY7) eBioscience Cat# 14-0443-82; RRID:AB_1633414 Phospho-S6 Ribosomal Protein (Ser235/236) − 488 Cell Signaling Cat# 4803; RRID: AB_916158 S6 Ribosomal Protein − 488 Cell Signaling Cat# 5317; RRID:AB_10694920 Anti-mouse CD31 eBiosciences Cat# 553370; RRID:AB_394816 Monoclonal CD34 (RAM34) eBioscience Cat# 14034182; RRID:AB_467210 Lectin (LEL, TL) DyLight 649 Vector Lab Cat# DL-1178-1 Biological samples BRAFV600K,PTPN11N58S National Cancer Institute 563396-261-R Chemicals, peptides, and recombinant proteins Linifanib (ABT869) MedChemExpress Cat# HY-50751 Vemurafenib (PLX4032) SellekChem Cat# S1267 CASIN MedChemExpress Cat# HY-12874 Deposited data RNA sequence data This paper GEO: GSE298488 Experimental models: Cell lines Human: WM3248 Coriell Medical Institute Cat# WC00081; RRID:CVCL_6798 Human: A375 ATCC Cat# CRL-1619; RRID:CVCL_0132 Human: cell-derived endothelial cells Lab of Hughes Hachey et al.56 Human: Lung Fibroblasts Lonza Cat# CC-2512 Experimental models: Organisms/strains NOD.Cg-PrkdcScid Jackson RRID:IMSR_JAX:005557 C57BL/6J Jackson RRID:IMSR_JAX:000664 Software and algorithms ImageJ National Institute of Health Bethesda USA https://imagej.nih.gov/ij/ AngioTool National Cancer Institute Zudire et al.51 neuTube Howard Hughes Medical Institute Feng L et al.52 REAVER BSD 3.0 open source Corliss et al.58 PANTHER https://pantherdb.org/ Mi H et al.75 STRINGv12 https://string-db.org/ Szklarczyk D et al.76 RUVSeq www.bioconductor.org/ Risso D et al.77 Samtools/1.15.1 Lab of Trapnell Li H et al.78 Prism 10.4.1 GraphPad www.graphpad.com/ HISAT2 v2.2.1 Lab of Trapnell Kim et al.79 FlowJo BD Bioscience https://www.flowjo.com/ MATLAB MathWorks www.mathworks.com/ products/matlab.html iScience 28, 112971, July 18, 2025 e1 ..

    Sample Prep:

    Article Title: Targeting USP2 regulation of VPRBP-mediated degradation of p53 and PD-L1 for cancer therapy
    Article Snippet: .. Methodology Sample preparation Instrument Software Cell population abundance EMT6 ATCC Cat# CRL-2755 293T ATCC Cat# CRL-3216; H1299 ATCC Cat# CRL-5803; U2OS ATCC Cat# HTB-96; CAL33 Creative Bioarray Cat# CSC-C0479; HUCCT1 Creative Bioarray Cat# CSC-C9200W; SKBR3 ATCC HTB-30; A549 ATCC Cat# CCL-185; A375 ATCC Cat# CRL-1619; H460 ATCC Cat# HTB-177; SKBR3 ATCC Cat# HTB-30; MDA-MB-435 ATCC Cat# HTB-129; MDA-MB-231 ATCC Cat# HTB-26; RM-1 ATCC Cat# CRL-3310; All cell lines were not authenticated. ..

    Multiple Displacement Amplification:

    Article Title: Targeting USP2 regulation of VPRBP-mediated degradation of p53 and PD-L1 for cancer therapy
    Article Snippet: .. Methodology Sample preparation Instrument Software Cell population abundance EMT6 ATCC Cat# CRL-2755 293T ATCC Cat# CRL-3216; H1299 ATCC Cat# CRL-5803; U2OS ATCC Cat# HTB-96; CAL33 Creative Bioarray Cat# CSC-C0479; HUCCT1 Creative Bioarray Cat# CSC-C9200W; SKBR3 ATCC HTB-30; A549 ATCC Cat# CCL-185; A375 ATCC Cat# CRL-1619; H460 ATCC Cat# HTB-177; SKBR3 ATCC Cat# HTB-30; MDA-MB-435 ATCC Cat# HTB-129; MDA-MB-231 ATCC Cat# HTB-26; RM-1 ATCC Cat# CRL-3310; All cell lines were not authenticated. ..



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    99
    ATCC paper n a a375 melanoma cells atcc crl 1619 a375
    Paper N A A375 Melanoma Cells Atcc Crl 1619 A375, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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